Part:BBa_K4574022

57aa-GS-Linker

GS-rich long linker tailored for joining the antigen-binding fragments of heavy and light chains in a single-chain fragment (scFab). It possesses the proper length and flexibility to optimise the functionality of the resultant scFab domain.

Source

The following basic part was developed as a flexible joint for scFab by Koerber et al.^[1].

The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ Koerber, J. T., Hornsby, M. J., & Wells, J. A. (2015). An Improved Single-Chain Fab Platform for Efficient Display and Recombinant Expression. Journal of Molecular Biology, 427(2), 576–586. https://doi.org/10.1016/j.jmb.2014.11.017

[1] Koerber, J. T., Hornsby, M. J., & Wells, J. A. (2015). An Improved Single-Chain Fab Platform for Efficient Display and Recombinant Expression. Journal of Molecular Biology, 427(2), 576–586. https://doi.org/10.1016/j.jmb.2014.11.017

[1]